2019-08-05 08:53:342024-12-31 18:32:07
description
ATP-dependent DNA helicase, acts together with [[protein|RecJ]]
ATP-dependent DNA helicase, acts together with [[protein|RecJ]] in replication fork maintenance
function
control of DNA recombination, maintenance of genomic stability
replication fork maintenance
The protein
Catalyzed reaction/ biological activity
unwinds covalently closed ds DNA (in complex with [[protein|SsbA|SSB]]) [pubmed|10360177]
[[protein|RecQ ]]controls [[protein|TopB|Topo III]] (de)catenation activity [pubmed|11497238,10360177]
unwinds covalently closed ds DNA (in complex with [[protein|SsbA|SSB]]) [pubmed|10360177]
[[protein|RecQ ]]controls [[protein|TopB|Topo III]] (de)catenation activity [pubmed|11497238,10360177]
ATP + H2O --> ADP + H+ + phosphate (according to UniProt)
Biological materials
Mutant
MGNA-B414 (yocI::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1413 NBRP B. subtilis, Japan]
BKE19220 (Δ[[gene|recQ]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE19220 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAACGTGTAAACCTCGCT, downstream forward: _UP4_TAAAAAAACAGAAAAACAAT
BKK19220 (Δ[[gene|recQ]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK19220 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAACGTGTAAACCTCGCT, downstream forward: _UP4_TAAAAAAACAGAAAAACAAT
GP3543 (Δ[[gene|recQ]]::kan), available in [SW|Jörg Stülke]'s lab
MGNA-B414 (yocI::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1413 NBRP B. subtilis, Japan]
BKE19220 (Δ[[gene|recQ]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE19220 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAACGTGTAAACCTCGCT, downstream forward: _UP4_TAAAAAAACAGAAAAACAAT
BKK19220 (Δ[[gene|recQ]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK19220 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAACGTGTAAACCTCGCT, downstream forward: _UP4_TAAAAAAACAGAAAAACAAT
References
Reviews
References
Original publications
The protein
[SW|Domains]
[SW|Helicase ATP-binding domain] (aa 27-196) (according to UniProt)
[SW|Helicase C-terminal domain] (aa 216-367) (according to UniProt)
HRDC domain (aa 513-591) (according to UniProt)
The protein
[SW|Localization]
associated with the [SW|replisome] [pubmed|17853894]